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背景:短暂全脑缺血再灌流早期海马各区域小白蛋白(parvalbumin,PV)阳性神经元的变化程度以及变化时间是否存在敏感性差异?目的:研究缺血敏感脑区海马的PV阳性神经元的改变以及缺血性脑损伤的细胞分子机制。设计:随机对照的实验设计。地点和对象:实验在中山大学中山医学院人体解剖学教研室完成。40只成年健康雄性大鼠,由中山大学中山医学院实验动物中心提供。干预:建立4管阻塞缺血大鼠模型,随机分为正常对照组,缺血再灌流0,6,12,24h共5组,每组8只。全脑缺血20min后再分别灌流0～24h。PV免疫组织化学反应观察海马各区域PV阳性神经元表达水平及定量PV阳性神经元数目。主要观察指标:海马各区域PV阳性神经元表达水平及定量PV阳性神经元数目。结果:正常对照组大鼠海马各区域(CA1,CA2,CA3)的始层、锥体层、放射层和腔隙分子层,齿状回的颗粒层、分子层及多形层(门区)的切片观察均出现PV阳性反应。再灌流6～12h内见PV阳性神经元出现胞体肿胀、胞浆染色变淡等变化。放射层突起密度减少;PV阳性终末的颗粒密度稀疏,以锥体细胞胞体周围的变化为明显。PV阳性神经元数目:缺血再灌流0h时,海马CA2区从(20.67±1.16)个减少为(11.66±3.06)个,CA3区从(12.33±2.52)个减少为(4.33±1.53)个;再灌流6h时,海马CA2区从(20.67±1.
BACKGROUND: In the early stage of transient global cerebral ischemia-reperfusion, the changes of parvalbumin (PV) -positive neurons in different regions of the hippocampus and whether there is a difference in the sensitivity of the changes in time. Objective: To study whether PV-positive neurons in hippocampus Changes and cellular molecular mechanisms of ischemic brain injury. Design: Randomized controlled experimental design. Location and Subjects: Experiments were performed at the Human Anatomy Department of Zhongshan Medical College of Sun Yat-sen University. Forty adult healthy male rats were provided by Experimental Animal Center of Zhongshan Medical College of Sun Yat-sen University. Intervention: The establishment of 4-tube occlusion model in rats, were randomly divided into normal control group, ischemia-reperfusion 0,6,12,24 h total of 5 groups of 8. Total cerebral ischemia 20min and then perfusion 0 ~ 24h. The expression of PV-positive neurons and the number of PV-positive neurons in hippocampus were observed by PV immunohistochemistry. MAIN OUTCOME MEASURES: PV-positive neurons in the hippocampus and the number of PV-positive neurons were quantified. Results: In the normal control group, the initial layer, pyramidal layer, radioactive layer and lacunar layer, the granular layer, the molecular layer and the polymorphic layer in the hippocampus (CA1, CA2 and CA3) The sections were observed PV positive reaction. During 6 ~ 12h of reperfusion, PV-positive neurons showed swelling of soma and staining of cytoplasm became lighter. The density of the radioactive layer was reduced; the density of PV-positive terminal particles was sparse, and the changes around the soma were obvious. The number of PV positive neurons decreased from (20.67 ± 1.16) to (11.66 ± 3.06) and (CA3) decreased from (12.33 ± 2.52) to (4.33 ± 1.53) at 0h after ischemia / reperfusion, respectively. At 6h after reperfusion, the hippocampal CA2 region decreased from (20.67 ± 1.