人类使用特异的个体型抗体,对于临床诊断和治疗是一项重要的措施.然而以抗体做为试剂,就其效能及特异性来说,仍有一定的问题,为此要广泛探讨人单克隆抗体,其方法很多,可用EBV激活的B细胞与人骨髓瘤细胞、鼠骨髓瘤细胞及人淋巴样母细胞杂交,建立稳定分泌抗体的克隆.人-人淋巴细胞融合不易成功,人淋巴细胞与鼠骨髓瘤细胞融合可产生杂种,但由于丢失人染色体,所以也不稳定.EBV激活的B细胞与 Human use of specific antibody is an important clinical diagnosis and treatment is an important measure.However, the use of antibodies as reagents, in terms of its efficacy and specificity, there are still some problems, for which to be widely discussed human monoclonal Antibodies, in many ways, can be EBV-activated B cells with human myeloma cells, murine myeloma cells and human lymphoblastoid cells to establish a stable antibody-secreting clone.Human-human lymphocyte fusion is not easy, human lymphocytes and Fusion of murine myeloma cells produces hybrids, but is also unstable due to the loss of human chromosomes. EBV-activated B cells and