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实验结果指出,在 NaN_3(10~(-3)M,种子予浸16小时),γ-射线(15KR)和快中子(1×10~(11)N_f/cm~2)三项处理中,六棱大麦“矮秆齐”M_1穗中皆有一部分叶绿素突变不能在 M_2代,而要迟至 M_3代才初次分离显现。当每个 M_2穗行出苗16—20株时,以 M_1穗为基础计算得到的这部分突变的突变频率在三种处理中分别为36.1,16.8和6.4%。这部分在M_2代隐而不现的叶绿素突变的突变频率与在M_2代显现的叶绿素突变频率(分别为28.8,11.6和9.5%)之和,即为总的叶绿素突变频率,它们在三种处理中分别为64.9,29.4和15.9%。由此可见在 M_2代隐而不现的叶绿素突变约占各处理中叶绿素突变总数的50%上下。这部分突变所以迟至M_3代才分离显现,除了 M_2代苗数这个限制因素以外,主要原因是它们的突变扇形体较小(绝大多数小于40%,其中不少小于10%)或嵌合体水平较低。M_3代初次显现的叶绿素突变的突变谱与 M_2代的无显著差异。
The experimental results show that in the three treatment of NaN3 (10-3M, seed pre-immersion for 16 hours), γ-ray (15KR) and fast neutron (1 × 10-11 Nf / cm2) , Six-row barley “dwarf Qi ” M_1 spike in a part of the chlorophyll mutation can not M_2 generation, and as late as M_3 generation was first isolated. When the number of M_2 spikes per plant was 16-20, the mutation frequency of M_1 spike was 36.1, 16.8 and 6.4% in the three treatments respectively. This part of the chlorophyll mutation in M_2 on behalf of the frequency of mutation and chlorophyll mutation frequency in the M_2 generation (respectively 28.8,11.6 and 9.5%), that is the total frequency of chlorophyll mutations in the three treatments Respectively, at 64.9, 29.4 and 15.9%. Thus we can see that the chlorophyll mutation hidden in the M_2 generations accounted for about 50% of the total number of chlorophyll mutations in each treatment. This part of the mutation was isolated as late as M_3, except for the limiting factors of the M_2 generation. The main reason for this mutation was that their mutant segments (the vast majority less than 40%, many less than 10%) or the chimera Low level. There was no significant difference between M_3 generation and chlorophyll mutation in M_3 generation.