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目的 观察着床前小鼠子宫内膜及胚卵整合素α4 、β1 mRNA的表达。 方法 用原位杂交技术检测妊娠 1～ 4d小鼠子宫内膜及胚卵整合素α4 、β1 mRNA的表达。 结果 整合素α4 、β1 mRNA主要表达在子宫内膜腔上皮 ,腺上皮 ,表达强弱不等。妊娠 3d基质细胞出现阳性信号 ,4d增强 ;胚卵整合素α4 、β1 mRNA仅在Ⅱ细胞期弱表达 ,在其他时期表达较强。 结论 妊娠 1～ 4d小鼠子宫内膜及胚卵持续表达整合素α4 、β1 mRNA ,提示它们可能参与小鼠胚泡的着床过程。
Objective To observe the expression of integrin α4 and β1 mRNA in the endometrium and embryo of mice before implantation. Methods The expressions of integrin α4 and β1 mRNA in endometrium and embryo of pregnant mice were detected by in situ hybridization. Results Integrin α4 and β1 mRNA were mainly expressed in endometrial luminal epithelium and glandular epithelium, with different expression intensity. The positive signal of stromal cells appeared on day 3 of gestation, which was enhanced on day 4. The integrin α4 and β1 mRNA of embryo eggs were only weakly expressed in the second phase and strongly expressed in other stages. Conclusions The expression of integrin α4 and β1 mRNA in the endometrium and embryo eggs of mice on day 1 to day 4 of pregnancy shows that they may participate in the implantation of mouse blastocysts.