论文部分内容阅读
目的：用低硒／维生素E(Se／VE)饲料喂养的大鼠动物模型，施以冰冰在刺激，观察Se，VE对心肌血管活性物质的影响。方法：用60只Wistar大鼠，按体重和性别随机分为低硒／维生素EI组（Se/VEDGI）及上述每kg饲料补硒（亚硒酸钠）0.1mg（Se／VEDGI＋Se）组、补a－VE100mg（Se／VEDGI＋VE）组、联合补Se，VE（Se／VEDGI＋Se／VE）组、低硒／维生素EII组（Se／VEDGII）和常规饲料组（SG)等六组。饲养70d，测定各组动物心肌内皮素（ET），一氧化氮（NO），去甲管上腺素（NE），血管紧张素II（Ang－II），单胶氧化酶（MAO－A），超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GSH－Px），脂褐素（lipofuscin）等水平。结果：Se／VEDG组心肌ET，Ang－II含量明显高于其它组；NO含量明显低于其它组；心肌NE水平增高，而特异性分解NE的MAO－A活性降低；心肌Lipofusein含量明显增加；SOD，GSH－Px含量降低。补Se组、补SE／VE组、低Se/VEII组和对照组上述指标活性明显改善。结论：冷刺激条件下硒和维生素E对大鼠心脏内血管活性物质有明显影响。
OBJECTIVE: To observe the effects of Se and VE on myocardial vasoactive substances in a rat model of feeding with low selenium / vitamin E (Se / VE) feed. Methods: Sixty Wistar rats were randomly divided into Se / VEDGI group (Se / VEDGI) and 0.1mg (Se / VEDGI + Se) selenium group Se / VEDGI + Se group, Se / VEDGI + Se / VE group, Se / VEDGII group and SG group. Animals were sacrificed for 70 days. The levels of endothelin (ET), nitric oxide (NO), norepinephrine (NE), angiotensin II (Ang-II) and monooxygenase (MAO-A) , Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), lipofuscin (lipofuscin) and other levels. Results: The contents of ET and Ang-II in myocardium of Se / VEDG group were significantly higher than those of the other groups; the content of NO was significantly lower than that of the other groups; the level of NE was increased while the activity of MAO-A in NE was decreased; SOD, GSH-Px content decreased. Supplement Se group, SE / VE group, low Se / VEII group and control group significantly improved the above indicators. CONCLUSION: Selenium and vitamin E have a significant effect on vasoactive substances in rat heart under cold stimulation.