目的 :胰岛素脂质体中胰岛素含量的测定。方法 :采用高效液相色谱法 ,色谱柱为 Hypersil ODS2 ,流动相为 0 .1mol L- 1磷酸二氢钠 - 0 .0 5 m ol L- 1硫酸钠 -乙睛 =36∶ 36∶ 2 8,流速为 1m l m in- 1 ,检测波长为 2 14 nm。结果 :高、中、低的回收率分别为99.5 5 % ,98.2 3% ,10 0 .9% ,RSD分别为 1.1% ,1.7%和 0 .6 1%。在 5～ 10 0μg m l- 1 测定范围内 ,线性良好 ,r=0 .9999。结论 :方法可靠 ,准确 Objective: Determination of insulin content in insulin liposomes. Methods: High performance liquid chromatography (HPLC) was performed on a Hypersil ODS2 column with a mobile phase of 0.1 mol L-1 sodium dihydrogen phosphate - 0.05 mol L-1 sodium sulfate-acetonitrile = 36:36:28 , The flow rate of 1m lm-1, detection wavelength of 2 14 nm. Results: The recoveries of high, medium and low were 99.5 5%, 98.2 3% and 109.9%, respectively. The RSDs were 1.1%, 1.7% and 0.61% respectively. In the range of 5 ~ 100μg m l-1, the linearity was good, r = 0.9999. Conclusion: The method is reliable and accurate