论文部分内容阅读
选用81种随机引物,利用RAPD技术对13种栽培大豆品种的基因组DNA多态性进行了分析,结果表明大多数的随机引物均得到了良好的PCR扩增结果,显示出不同的栽培大豆品种之间基因组DNA既有高度的同源性,又具有良好的多态性.通过Nei氏遗传共享度分析,使用UPGMA(theunweightedpairgroupmethodforarithmeticaverages)进行聚类分析,得到了UPG-MA系统树和实验材料之间的遗传关系.
Eighty-one random primers were used to analyze the genomic DNA polymorphisms of thirteen cultivated soybean cultivars by RAPD. The results showed that most of the random primers obtained good PCR amplification results, which showed that different varieties of cultivated soybean Between the genomic DNA both highly homologous, but also has a good polymorphism. By Nei's genetic sharing analysis, UPGMA (theunweightedpairgroupmethodforarithmeticaverages) was used for cluster analysis, and the genetic relationship between the UPG-MA phylogenetic tree and the experimental materials was obtained.