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为寻求较理想的动物乙型肝炎模型，在研制成功广州麻鸭乙型肝炎模型基础上，比较先天和后天感染鸭乙肝病毒的广州麻鸭外周血中病毒血症的差异。方法是采用斑点杂交方法（ｄｏｔｂｌｏｔ）检测了广州麻鸭先天和后天感染鸭乙型肝炎病毒（ＤＨＢＶ）后外周血中ＤＨＢＶ－ＤＮＡ的动态。结果：先天感染的广州麻鸭外周血中病毒血症水平较高，出生后（即感染后）２６ｄ时ＤＨＢＶ－ＤＮＡ下降到一较低的水平，７２ｄ时仍维持较低水平。出生后１ｄ感染ＤＨＢＶ的广州麻鸭，感染后９ｄ时血中开始出现低水平的ＤＨＢＶ－ＤＮＡ，１１ｄ时达到高峰，之后逐渐下降，２６ｄ时下降到一较低的水平，到７２ｄ时仍维持低ＤＨＢＶ－ＤＮＡ水平。并采用后天感染ＤＨＢＶ的广州麻鸭作为鸭乙肝模型筛选抗乙肝中草药，以无环鸟苷为阳性对照药，蒸馏水为阴性对照药，发现每次阳性对照组和阴性对照组均成立，证明该模型重复性良好。并发现台湾单纯叶下珠、虫草和树舌均有降低ＤＨＢＶ－ＤＮＡ的作用。
In order to find a better animal model of hepatitis B, based on the successful development of the model of hepatitis B in Guangzhou ducks, we compared the difference of viremia in peripheral blood of Guangzhou ducks who were infected by duck and hepatitis B virus. Dotblot was used to detect the dynamic changes of DHBV-DNA in peripheral blood of ducks infected with duck hepatitis B virus (DHBV) after congenital and acquired day of Guangzhou mallard ducks. Results: The level of viremia in the peripheral blood of congenital infected Guangzhou duck was higher than that of the control group. DHBV-DNA decreased to a lower level at 26 days after birth (ie, after infection), and remained low at 72 days. DHBV infected DHBV at 1 d after birth showed low levels of DHBV-DNA in blood at 9 days after infection, reaching a peak at 11 d and then gradually decreasing. It dropped to a lower level at 26 d and remained low at 72 d DHBV-DNA levels. And the use of Guangzhou DHBV infected ducks infected with DHBV duck model as anti-hepatitis B Chinese herbal medicine screening to acyclovir as a positive control drug, distilled water as a negative control drug found that each positive control group and negative control group were established that the model Repeatability is good. And found that Taiwan simply Phyllanthus, Cordyceps and tree tongue have reduced the role of DHBV-DNA.