HBsAg上亚型和组特异决定簇的抗体结合部位已被鉴定。作者对T细胞应答是否针对抗体特异性的相同分子内区域作了检测。利用体外T细胞培养技术,取免疫小鼠输出淋巴结细胞和接种疫苗者外周血单核细胞,与一系列HBsAg相关抗原共同培养,以~3HdThd掺入量测定T细胞的增殖。以天然HBsAg、多肽结构亚单位(P-25)、P-25的 Antibody binding sites for HBsAg isoform and group specific determinants have been identified. The authors tested whether T cell responses are directed against the same intramolecular region of antibody specificity. Using in vitro T cell culture technology, we took the lymphocytes from immunized mice and the peripheral blood mononuclear cells from the vaccinated mice and co-cultured them with a series of HBsAg-related antigens. The proliferation of T cells was determined by ~ 3HdThd incorporation. With native HBsAg, polypeptide structural subunits (P-25), P-25