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为探讨甲醛的氧化应激损伤效应,采用V79细胞株作为实验材料,以0、50、100、200、400、800、1600μmol/L的液态甲醛对细胞染毒,1h后按照试剂盒说明书检测细胞总超氧化物歧化酶(SOD)活力、总一氧化氮合酶(NOS)活力以及丙二醛(MDA)含量。实验表明,液态甲醛可以使V79细胞的总SOD活力和总NOS活力呈现下降趋势,200μmol/L及以上浓度组与对照组相比下降更为显著(P<0.05),而MDA含量则呈上升趋势,400μmol/L及以上浓度组与对照组相比升高更加明显(P<0.05)。本实验条件下,较高浓度的甲醛可以导致细胞的氧化应激损伤。
In order to investigate the oxidative stress injury effect of formaldehyde, V79 cell line was used as the experimental material, and the cells were exposed to liquid formaldehyde at 0, 50, 100, 200, 400, 800 and 1600 μmol / L for 1 hour, Total superoxide dismutase (SOD) activity, total nitric oxide synthase (NOS) activity and malondialdehyde (MDA) content. The results showed that the liquid formaldehyde could decrease the total SOD activity and the total NOS activity of V79 cells, and the concentration of 200 μmol / L and above decreased more significantly than that of the control group (P <0.05), but the MDA content increased , 400μmol / L and above concentration group compared with the control group increased more significantly (P <0.05). Under the experimental conditions, higher concentration of formaldehyde can lead to cell oxidative stress injury.