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除抗体滴度外,抗体对细菌的活性(Avidity)亦是评价其生物学效能的重要参数。本实验目的在于研究成人牙周炎(AP)患者血清中IgG抗体对P.g各种表面抗原的活性。 选取P.g非毒性株AICC33277及毒性株16-1,分别制备全细胞抗原、LPS及表面纤毛抗原,采用ELISA及乙二胺分解(diethylamine dissociation)ELISA法分别测定AP患者治疗前后血清IgG滴度及其对不同抗原的活
In addition to the antibody titer, the antibody's activity on bacteria (Avidity) is also an important parameter to evaluate its biological performance. The purpose of this experiment was to investigate the activity of IgG antibodies in serum of adult periodontitis (AP) patients against various surface antigens of P.g. The Pg non-toxic strain AICC33277 and the virulent strain 16-1 were selected to prepare whole cell antigen, LPS and surface ciliated antigens respectively. The serum IgG titers of AP patients before and after treatment were measured by ELISA and diethylamine dissociation ELISA Live for different antigens