论文部分内容阅读
目的：研究环腺苷酸 (cyclic adenosine monophosphate， cAMP)、整合素（ integrinα 3）在三磷酸腺苷 (adenosin triphosphate， ATP)作用下对人胃癌细胞系（ MGC- 803）恶性表型的去恶化作用。方法：应用 ATP作用于人胃癌细胞，①每日计数细胞计算抑制率和绘制生长曲线； ②软琼脂集落形成法检查集落形成能力； ③放免测定法和电镜细胞化学方法检查 cAMP-磷酸二酯酶（ cAMP- phosphodiesterase， cAMP- PDEase）活性的改变； ④放免测定法和免疫细胞化学方法检测 cAMP水平的变化；⑤免疫细胞化学方法检测整合素和纽蛋白的表达。结果：① ATP抑制 MGC- 803细胞的增殖和在软琼脂内形成集落的能力，以及 cAMP- PDEase活性； ② ATP提高 MGC- 803细胞内 cAMP水平和增强细胞的整合素和粘着斑－纽蛋白的表达。结论： ATP抑制 MGC- 803细胞增殖并诱导分化； cAMP、整合素和纽蛋白在 ATP对 MGC- 803细胞的恶性表型去恶化过程中起重要的信号调节作用。
Objective: To study the degenerative effects of cyclic adenosine monophosphate (cAMP) and integrin α 3 on the malignant phenotype of human gastric cancer cell line (MGC-803) under the action of adenosin triphosphate (ATP). METHODS: ATP was used to treat human gastric cancer cells. 1 The daily cell count was used to calculate the inhibition rate and the growth curve was drawn. 2 The soft colony colony formation method was used to examine the colony formation ability. 3 The radioimmunoassay and electron microscopy cytochemistry were used to examine the cAMP-phosphodiesterase. Changes in cAMP-phosphodiesterase (cAMP-PDEase) activity; 4 Changes in cAMP levels detected by radioimmunoassay and immunocytochemistry; 5 Immunocytochemistry for detection of integrin and vinculin expression. RESULTS: 1 ATP inhibited the proliferation of MGC-803 cells and the formation of colonies in soft agar, as well as the cAMP-PDEase activity; 2 ATP increased cAMP levels in MGC-803 cells and enhanced the integrins and focal adhesions of neurons. expression. Conclusion: ATP can inhibit the proliferation of MGC-803 cells and induce differentiation. cAMP, integrin and vinculin play an important role in the regulation of malignant phenotype depletion of MGC-803 cells.