建立转bcl xl基因小鼠 ,继而传代建系 ,用于缺血性脑血管疾病的研究。采用显微注射法 ,将人bcl xl基因注入昆明小白鼠受精卵获得子代鼠 ,然后作PCR ,Southern blot ,mRNA ,Western blot检测以获得阳性鼠。实验中注射受精卵 16 5 4枚 ,移植卵数 14 4 8枚 ,受体鼠 4 9只 ,怀孕鼠 7只 ,子代鼠 13只 ,整合数 4只并均有表达 ,植入受精卵的存活率 83% ,受精卵的总存活率 0 .78% ,移植鼠的怀孕率 14 .3% ,整合效率 30 .7% ,总有效率为 0 .2 4 %。初步获得了转bcl xl基因小鼠 Establishment of transgenic mice bcl xl, followed by passage of the Department for the study of ischemic cerebrovascular disease. The human bcl xl gene was injected into Kunming mice fertilized eggs by microinjection method to obtain progeny mice, then PCR, Southern blot, mRNA, Western blot detection to obtain positive mice. In the experiment, 16 5 4 fertilized eggs, 14 4 8 transplanted eggs, 49 recipient mice, 7 pregnant rats, 13 offspring mice, 4 integrative animals were all injected with fertilized eggs The survival rate was 83%, the total survival rate of fertilized eggs was 0.78%, the pregnancy rate of transplanted mice was 14.3%, the integration efficiency was 30.7% and the total effective rate was 0.24%. Preliminary obtained bcl xl transgenic mice