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目的:观察GDNF启动子1区在人脑胶质瘤细胞中的甲基化修饰状态,以期探讨其对于GDNF在胶质瘤中高表达的影响。方法:基因测序检测10例胶质瘤与5例正常脑组织中GDNF基因序列,比较其基因是否有突变发生;重亚硫酸盐修饰后基因测序检测20例胶质瘤(10例低级别和10例高级别)与5例正常脑组织中GDNF启动子1区甲基化修饰状态。结果:GDNF启动子1区基因在胶质瘤中没有发生突变;GDNF启动子1区甲基化修饰在正常脑组织、低级别、高级别中发生率分别为72.25%、86.25%、86.75%。在胶质瘤中的甲基化修饰水平比正常脑组织明显增高(P<0.05),而高低级别之间无显著性差异。结论:在胶质瘤细胞中,GDNF启动子1区发生了高甲基化修饰,这种修饰很可能会影响GDNF基因的表达。
OBJECTIVE: To observe the methylation status of GDNF promoter 1 in human glioma cells in order to investigate its effect on GDNF overexpression in gliomas. Methods: GDNF gene sequences were detected in 10 gliomas and 5 normal brain tissues by gene sequencing, and whether there was a mutation in GDNF gene was compared with it; 20 cases of gliomas were detected by sequencing of bisulfite modified gene (10 cases of low grade and 10 cases of low grade Cases of high level) and 5 cases of normal brain tissue GDNF promoter 1 methylation status. Results: The gene of GDNF promoter region 1 did not change in glioma. The methylation of GDNF promoter promoter in region 1 was 72.25%, 86.25% and 86.75% respectively in normal brain tissue, low grade and high grade. The level of methylation in glioma was significantly higher than that in normal brain tissue (P <0.05), but there was no significant difference between high and low grade. CONCLUSION: In glioma cells, hypermethylation of GDNF promoter region 1 occurs, which may affect the expression of GDNF gene.