目的:考察pH、温度、光照等因素对胸腺肽α1稳定性的影响。方法:采用HPLC法分析胸腺肽α1在不同条件下的含量变化,将浓度变化与时间作线性回归,求得药物在不同pH条件下的降解曲线方程,利用降解速率常数反映出胸腺肽α1在恒温条件下的最适pH。结果:酸、碱、光照及高温均能促进胸腺肽α1的降解,药物的最稳定pH在5～6之间,且在醋酸盐缓冲液中较稳定。结论:温度、pH、光照等因素对胸腺肽α1的稳定性有显著影响。 Objective: To investigate the effects of pH, temperature, light and other factors on the stability of thymosin α1. Methods: Thymosin α1 was assayed by HPLC under different conditions. The linear relationship between the concentration and time was obtained. The degradation equation of the drug was obtained under different pH conditions. The degradation rate constant of thymosin α1 under constant temperature The optimum pH. Results: Acid, alkali, light and high temperature can promote the degradation of thymosin α1. The most stable pH value of drug is between 5 and 6, and it is more stable in acetate buffer. Conclusion: Temperature, pH, light and other factors have a significant impact on the stability of thymosin α1.