以 NBT/VB2 /蛋氨酸为 O· - 2 产生、检测体系 ,对自制低聚壳聚糖及其金属配合物进行了抗 O· - 2 活性研究 ,结果显示低聚壳聚糖及其金属配合物对 O· - 2 均具有明显的清除活性 .质量浓度为 0 .5× 1 0 - 2 g/m L时 ,壳聚糖对 O· - 2 的清除率达 80 .3 % ,其与氯化镧、醋酸铜、醋酸钴的配合物对 O· - 2 的清除率分别为98.9%、84.1 %、78.4% ;相同条件下 ,高分子壳聚糖和单糖对 O· - 2 没有明显的清除作用 ,清除率仅为 1 3 %、9.5 % .随着样品质量浓度降低 ,低聚壳聚糖及其金属配合物的清除活性逐渐下降 With NBT / VB2 / methionine as O · 2, the system was tested and the anti-O · 2 activity of self-made chitosan and its metal complexes was studied. The results showed that the chitosan and its metal complexes Had clear scavenging activity on O · 2. The scavenging rate of O · 2 to chitosan reached 80.3% at mass concentration of 0.5 × 10-2 g / ml, The scavenging rates of O · 2 to the complexes of lanthanum, copper acetate and cobalt acetate were 98.9%, 84.1% and 78.4%, respectively. Under the same conditions, the macromolecule chitosan and monosaccharide did not clear O · 2 obviously , The clearance rate was only 13%, 9.5% .With the sample concentration decreased, the scavenging activity of oligomeric chitosan and its metal complexes gradually decreased