荧光图象漂白技术原理的基本要点是:在显微镜的光路中插入掩膜(光栅),经强激光瞬时漂白,使细胞表面上荧光标记分子产生不可逆的光化学漂白而形成有规则的荧光图象,随即通过SIT(硅增强靶)摄象管,用微弱的激光束监测荧光图象的变化,照相机速度摄取荧光图象的变化,由此观察和研究细胞表面分子的二维变化及其运动规律。我们研制成激光漂白荧光图象 The basic principle of fluorescence image bleaching technology is that a mask (grating) is inserted into the optical path of the microscope and is instantaneously bleached by a strong laser so as to cause irreversible photochemical bleaching of the fluorescently labeled molecules on the cell surface to form a regular fluorescent image, Fluorescence images were monitored with a faint laser beam through a SIT (silicon enhanced target) camera tube. Changes in fluorescence images were captured by camera speed to observe and study the two-dimensional changes in cell surface molecules and their movement patterns. We developed a laser bleached fluorescent image