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研究光果甘草中光甘草定的提取工艺。在单因素实验的基础上,以100%工业甲醇为提取溶剂,选定料液比、提取时间和提取温度3个因素的3个水平进行中心组合实验,建立光甘草定得率和纯度的二次回归方程,其决定系数分别为98.08%和91.21%。通过响应面及岭嵴分析得到优化的提取工艺条件是:料液比1:38.5g/mL、提取时间64min、提取温度50.4℃,在该条件下光甘草定得率预测值为0.52%,验证值为0.51%。
Study on the light of licorice light glycyrrhizin extraction process. On the basis of single factor experiment, the central combination experiment was conducted with three levels of three factors: extraction solvent, 100% industrial methanol, extraction time and extraction temperature, to establish the second The regression equation has a coefficient of determination of 98.08% and 91.21% respectively. The optimal conditions for the extraction process were obtained as follows: the ratio of material to liquid was 1: 38.5 g / mL, the extraction time was 64 min and the extraction temperature was 50.4 ℃. The predicted yield of K. licheniformis under this condition was 0.52% The value is 0.51%.