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考察不同浓度、不同种类的稳渗剂及不同浓度的酶液对紫杉醇产生菌的原生质体产率的影响;不同种类的稳渗剂对原生质体再生的影响。结果表明,5%的酶液浓度,0.5mol/L KCl为稳渗剂原生质体产率最佳;以0.8mol/L kcl为稳渗剂原生质体的再生率最佳。
The effects of different concentrations of different types of stabilizers and different concentrations of enzyme solution on the yield of paclitaxel-producing bacteria were investigated. The effects of different types of stabilizers on the regeneration of protoplasts were investigated. The results showed that the yield of protoplast was the best when the concentration of enzyme solution was 5% and 0.5mol / L KCl was the stabilizing agent. The regeneration rate of protoplast was the best with 0.8mol / L kcl as stabilizing agent.