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目的:观察油酸型急性肺损伤(AIL)大鼠肺组织中小G蛋白(RhoA)的表达以及其对IL-8/IL-10表达的影响,探讨RhoA与急性肺损伤的关系。方法:建立大鼠油酸急性肺损伤模型,检测各组大鼠肺湿/干重比,比较肺组织病理学改变;采用聚合酶链反应检测肺组织匀浆RhoA表达,酶联免疫吸附(ELISA)法测定大鼠IL-10/IL-8的变化。结果:肺W/D损伤组在2、6、12、24、48 h均较对照组明显升高(P<0.01),而治疗组在相同时间点较损伤组降低(P<0.05)。治疗组2、6、12、24、48 h血液中IL-10较对照组升高(P<0.05);IL-10较损伤组同时间点明显升高(P<0.05)。损伤组各时间点血清IL-8较对照组明显升高(P<0.01),而干预组较损伤组同时间点显著降低(P<0.01)。损伤组2、6、12、24、48 h大鼠肺组织RhoA较对照组明显升高(P<0.01);干预组与损伤组同时间点比较无显著差异(P>0.05)。结论:油酸型急性肺损伤大鼠肺组织中RhoA表达增加,而RhoA表达增加可致大鼠肺损伤程度增大,RhoA可能通过上调表达IL-8及下调IL-10表达作用而导致肺损伤程度加大。
Objective: To observe the expression of small G protein (RhoA) and its effect on the expression of IL-8 / IL-10 in lung tissue of oleic acid-induced acute lung injury (AIL) rats and to explore the relationship between RhoA and acute lung injury. Methods: The model of acute lung injury induced by oleic acid in rats was established. The wet / dry weight ratio of lungs of rats in each group were measured and the pathological changes of lung tissues were compared. The expression of RhoA in lung homogenates was detected by polymerase chain reaction (ELISA) ) Method to determine the changes of IL-10 / IL-8 in rats. Results: The lung W / D injury group was significantly higher than the control group at 2, 6, 12, 24 and 48 hours (P <0.01), while the treatment group was lower than the injury group at the same time point (P <0.05). The levels of IL-10 in the blood of the treated group were significantly higher than those of the control group at 2,6,12,24,48 h (P <0.05). The levels of IL-10 in the blood were significantly increased at the same time point (P <0.05). Serum IL-8 levels at each time point in the injury group were significantly higher than those in the control group (P <0.01), while those in the intervention group were significantly lower than those in the injury group at the same time point (P <0.01). RhoA in the lung tissue of rats in the injury group at 2, 6, 12, 24 and 48 h was significantly higher than that in the control group (P <0.01). There was no significant difference between the intervention group and the injury group at the same time point (P> 0.05). Conclusion: The expression of RhoA in lung tissue of rats with oleic acid-induced acute lung injury increased, while the expression of RhoA increased the degree of lung injury in rats. RhoA may induce lung injury by up-regulating the expression of IL-8 and down-regulating the expression of IL-10 Increased degree.