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体外培养大鼠前脂肪细胞,分别以2μmol/L、4μmol/L、8μmol/L的翅果油甾醇对其进行处理。采用MTT比色法检测细胞增殖情况;油红O染色化学比色法定量分析细胞内脂肪生成及细胞分化程度;逆转录-聚合酶链式反应(RT-PCR)分析过氧化物酶增殖物激活受体γ2(PPARγ2)mRNA表达情况,探讨翅果油甾醇对前脂肪细胞增殖分化的影响及其可能的机制。结果显示:各组细胞MTT测试OD值均低于对照组,4μmol/L及8μmol/L组细胞油红O染色的OD值及PPARγ2mRNA的表达量均显著下降。
Rat preadipocytes were cultured in vitro and treated with 2 μmol / L, 4 μmol / L, 8 μmol / L samara sterylol respectively. MTT assay was used to detect cell proliferation; oil red O staining chemical quantitative analysis of intracellular adipogenesis and cell differentiation; reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of peroxisome proliferator-activated Receptor γ2 (PPARγ2) mRNA expression, to explore the samara oil sterol on the proliferation and differentiation of preadipocytes and its possible mechanism. The results showed that the OD value of MTT assay in each group was lower than that in the control group. The OD value and PPARγ2 mRNA expression of the cells in 4μmol / L and 8μmol / L groups were significantly decreased.