论文部分内容阅读
背景与目的筛选肺鳞癌患者淋巴转移差异表达基因群。方法原发癌组织及区域淋巴结取自10例接受完全性肺癌切除手术的肺鳞癌患者。根据组织来源将标本分为三组:不伴淋巴转移的肺鳞癌组织(TxN-,n=5)、伴有淋巴转移的肺鳞癌组织(TxN+,n=5)及相应转移淋巴结中的肺鳞癌细胞(N+,n=5)。对各组进行激光显微切割以获得纯净癌细胞,T7RNA线性扩增获取足够量的RNA,实验通道和参照通道分别标记以后与含6000个已知人类基因或表达序列标签的cDNA基因芯片杂交,扫描荧光信号以后进行数据分析。结果共有37个基因可将TxN+组与TxN-组区分开,其中在TxN+组高表达的基因有8个,主要涉及蛋白合成、信号传导、伴侣蛋白和酶等。有29个基因在TxN-组高表达,这些基因主要编码细胞周期调节子、转导子、信号传导蛋白以及细胞凋亡调节蛋白。比较N+组与TxN+组却没有发现具有显著性的差异表达基因。结论肺鳞癌的淋巴转移表型的获得可能是早期事件。这些差异基因的发现有助于阐明肺鳞癌淋巴转移的分子机制和寻找新的治疗靶点。
BACKGROUND & AIM: To screen differentially expressed genes in patients with squamous cell carcinoma of the lung. Methods Primary cancer tissues and regional lymph nodes were obtained from 10 patients with lung squamous cell carcinoma undergoing complete lung resection. The specimens were divided into three groups according to their origin: lung squamous cell carcinoma without lymph node metastasis (TxN-, n = 5), squamous cell carcinoma with lymph node metastasis (TxN +, n = 5) and corresponding lymph node metastasis Lung squamous carcinoma cells (N +, n = 5). Laser microdissection of each group to obtain pure cancer cells, T7RNA linear amplification to obtain a sufficient amount of RNA, experimental channel and reference channel were labeled with 6000 known human genes or cDNA sequence tag expression of cDNA microarray, After scanning the fluorescence signal for data analysis. Results A total of 37 genes could distinguish TxN + group from TxN- group. There were 8 genes highly expressed in TxN + group, which mainly involved in protein synthesis, signal transduction, chaperonin and enzyme. Twenty-nine genes are highly expressed in the TxN-group, and these genes primarily encode cell cycle regulators, transducers, signaling proteins, and apoptosis regulatory proteins. Compared with N + group and TxN + group, no significant differentially expressed genes were found. Conclusion Obtaining of lymphatic metastasis phenotype in lung squamous cell carcinoma may be an early event. The discovery of these differentially expressed genes can help elucidate the molecular mechanism of lymphatic metastasis in lung squamous cell carcinoma and search for new therapeutic targets.