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目的观察新霉素（ＮＭ）对五肽促胃液素（ＰＧ）促人结肠癌细胞株ＳＷ４８０增殖的影响，探讨肌醇脂质信号通路可能参与的作用及临床意义．方法ＭＴＴ比色分析法检测活细胞数（ＶＣＣ）；［３Ｈ］肌醇标记细胞进行三磷酸肌醇（ＩＰ３）分析；Ｃａ２＋荧光测定技术检测细胞内Ｃａ２＋浓度；［γ３２Ｐ］ＡＴＰ掺入法测定蛋白激酶Ｃ（ＰＫＣ）活性．结果ＰＧ＋ＮＭ组的ＳＷ４８０细胞活细胞数降低，与对照组相比Ｐ＜００１，与ＰＧ组相比Ｐ＜００１；ＰＧ＋ＮＭ组细胞内ＩＰ３，Ｃａ２＋浓度降低，与ＰＧ组相比Ｐ＜００１，与对照组相比Ｐ＞００５；ＰＧ＋ＮＭ组膜ＰＫＣ活性降低，与ＰＧ组相比Ｐ＜００５，与对照组相比Ｐ＞００５．结论ＮＭ可能通过肌醇质脂信号通路而抑制ＰＧ促人结肠癌细胞株ＳＷ４８０的增殖，这将为结肠癌的抗信息传导治疗提供实验依据．
Objective To observe the effect of neomycin (NM) on the proliferation of human colon cancer cell line SW480 induced by pentagastrin gastrin (PG), and to explore the possible role and clinical significance of inositol lipid signaling pathway. Methods MTT assay was used to measure the number of viable cells (VCC); [3H]-inositol-labeled cells were used for inositol triphosphate (IP3) analysis; Ca2+ fluorescence assay was used to detect the intracellular Ca2+ concentration; [γ-32P] ATP was incorporated. The method measures protein kinase C (PKC) activity. Results The number of viable cells of SW480 cells in the PG+NM group decreased, compared with the control group P<001, compared with the PG group P<001; the PG+NM group decreased the intracellular IP3, Ca2+ concentration, compared with the PG group P< 0 01, compared with the control group P> 0 05; PG + NM group membrane PKC activity decreased compared with PG group P <0 05, compared with the control group P> 0 05. Conclusion NM may inhibit the proliferation of human colon cancer cell line SW480 through inositol lipoprotein signaling pathway, which will provide experimental basis for anti-information conduction therapy of colon cancer.