论文部分内容阅读
为了阐明已分离的盐碱适应性相关基因(Liuetal, 2000)的表达特性,以14d叶龄的水稻品种日本晴幼苗为材料,分别经NaHCO3 (60mmol/L,pH8. 50) )处理6h、12h、24h、48h, 及不同pH值(pH4. 50, 6. 50, 8. 50, 10. 50)的NaCl(100mmol/L), NaHCO3 (60mmol/L)和Na2CO3 (30mmol/L)处理24h, 以分析时间效应及浓度与pH值间的联合效应. 结果表明,与对照(H2O, pH6. 50)相比较,NaHCO3 (60mmol/L)处理6h、12h、24h、48h后,mitochondrialAT Pase6×103的转录本分别提高2. 73、0. 82、0. 01和1. 79倍;cAPX转录本在24h和48h较对照提高1. 17和1. 49倍;处理6h后CA和HSP90基因表达受显著抑制;UDPG-GT增强表达的滞后期为6h;thioredoxin受NaHCO3轻微抑制.不同pH值和24h处理条件下,NaHCO3和Na2CO3显著促进mitochondrialATPase6×103基因表达.CA基因表达受Na+、HCO-3 和pH的共同影响;而thioredoxin主要受Na+和pH的双重影响; cAPX也表现出类似趋势.UDPG-GT在Na2CO3处理中表达量最高,HSP90的表达随pH的升高而降低. 图6表2参13
In order to elucidate the expression characteristics of the genes related to salinity-alkalinity adaptation (Liu et al., 2000), the seedlings of 14-day-old Japonica seedlings were treated with NaHCO3 (60mmol / L, pH8. 50) for 6h, 12h, 24h, 48h and NaCl (100mmol / L), NaHCO3 (60mmol / L) and Na2CO3 (30mmol / L) with different pH values (pH4.55, The time effect and the combined effect of concentration and pH value were analyzed.The results showed that compared with the control (H2O, pH6. 50), the transcription of mitochondrial ATPase 6 × 103 was inhibited by NaHCO3 (60mmol / L) for 6h, 12h, 24h and 48h This increased by 2.73,0.82,0. 01 and 1.79 times; cAPX transcripts increased by 1.17 and 1.49 times at 24h and 48h compared with the control; After 6h treatment, the expression of CA and HSP90 gene was significantly inhibited ; The delayed expiration of UDPG-GT enhanced expression was 6h; thioredoxin was slightly inhibited by NaHCO3. NaHCO3 and Na2CO3 significantly promoted the expression of mitochondrial ATPase 6 × 103 under different pH and 24h treatment.CA gene expression was regulated by Na +, HCO-3 and pH While thioredoxin was mainly affected by Na + and pH, cAPX also showed a similar trend.UDPG-GT was the highest expression in Na2CO3, It was reduced with the increase of pH. FIG. 2 reference 13 in Table 6