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为了快速制作适合光学显微镜下观测的棉花叶片气孔切片，本研究采用不同方法对棉花叶片气孔进行切片制作，观察气孔的形态、数量、长度、宽度等形态特征。结果表明：用指甲油涂抹印迹法、透明胶带粘贴法都能够得到不同清晰程度的气孔切片，但这两种方法在操作过程中存在着撕取难、易移位等缺点。本研究针对以上缺点对两种方法进行了综合改进，改进后的综合法能够克服撕取难和易移位的不足，更快速地得到清晰的棉花叶片气孔切片，为棉花及其它植物叶片气孔切片制作提供了方法和技术支持。“,”In order to develop a simple and fast stomatal slice preparation technique, different methods were used to prepare stomatal slice of Gossypium hirsutum L. for observation of its morphological features, such as number, length, width and so on. The result showed that different methods can obtain some level of definition of leaf stomatal structure. Compared with other methods, synthetic method could get more clear specimens than other methods with simplicity of operation, rapidity and suitability for other plants, it provides a technique support for stomatal slice production of other plant species.