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分别用生物素肼化学标记和ＤＩＧ标记我国柑桔裂皮病类病毒（ＣＥＶｄ）全长克隆ｃＤＮＡ，用以上探针对不同来源的核酸样品进行斑点杂交。两种探针可检出病柑桔总核酸的最低含量约为４００ｎｇ／斑点和８０ｎｇ／斑点；生物素肼标记ＣＥＶｄ－ｃＤＮＡ探针，可检出ＣＥＶｄ－ｃＤＮＡ的最低含量约为１０ｐｇ／斑点。研制的ＣＥＶｄ检测试剂盒能检测出发病和隐性带毒苗木中的ＣＥＶｄ，灵敏、特异、简便、快速、试剂盒已被用于检测来自我国主要裂皮病病区、湖南、湖北、福建等地的柑桔和香橼材料。
The full-length cDNA of citrus splitting dermis virus (CEVd) was labeled with biotin hydrazine chemical labeling and DIG, respectively. The above probes were used for speckled hybridization of nucleic acid samples from different sources. The minimum detectable total citrus orange nucleic acid content of the two probes was about 400 ng / spot and 80 ng / spot; for the biotin hydrazine labeled CEVd-cDNA probe, the lowest detectable level of CEVd-cDNA was about 10 pg / spot. The developed CEVd test kit can detect CEVd in the pathogenic and recessive virulent seedlings, which is sensitive, specific, simple and rapid. The kit has been used to detect the disease from the main split disease area in our country, such as Hunan, Hubei, Fujian, etc. Ground citrus and citron materials.