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目的:探讨液化石油气急性中毒对小鼠海马齿状回及侧脑室室管膜下区神经发生的影响。方法:选择健康成年昆明小鼠随机分三组,分别为对照组,液化石油气(LPG)高、低剂量中毒组。使用密闭中毒箱行染毒实验。用Morris水迷宫训练测试其空间学习与记忆能力;腹腔注射5-溴脱氧尿苷(BrdU)后通过免疫组织化学方法来显示BrdU阳性细胞。结果:两个中毒组动物的学习记忆能力均弱于对照组(P<0.01),齿状回和室管膜下区内BrdU阳性细胞数目也低于对照组(P<0.01)。结论:液化石油气急性中毒通过抑制齿状回和室管膜下区的神经发生,影响小鼠的学习记忆能力和脑损伤后修复。
Objective: To investigate the effects of acute liquefied petroleum gas (LPG) poisoning on the neurogenesis in the hippocampal dentate gyrus and lateral subependymal zone in mice. Methods: Healthy adult Kunming mice were randomly divided into three groups: control group, high and low dose LPG poisoning group. The use of airtight poison box poisoning experiment. Morris water maze training was used to test the spatial learning and memory ability. BrdU positive cells were detected by immunohistochemistry after intraperitoneal injection of 5-bromodeoxyuridine (BrdU). Results: The learning and memory abilities of the two poisoning groups were weaker than those of the control group (P <0.01). The numbers of BrdU positive cells in the dentate gyrus and subependymal zone were also lower than those in the control group (P <0.01). Conclusion: The acute poisoning of liquefied petroleum gas (LPG) can affect the learning and memory ability and repair of brain injury by inhibiting the neurogenesis of dentate gyrus and subventricular zone.