研究了 Co( ) - PAR在吐温 80 -硫酸铵体系中的最佳萃取条件 ,建立了生物样品中测定痕量钴的非有机溶剂液 -固萃取光度法。在 p H7.0～ 1 0 .5的NH3· H2 O- NH4 Cl缓冲溶液中 ,金属配合物 Co( ) - PAR可被吐温 80固相富集 ,最大吸收波长为 51 5nm,钴含量在 0～ 5.0 μg/m L范围内服从比尔定律。可用于头发、茶叶和维生素 B12 针剂中痕量钴的测定 The optimum extraction conditions of Co () - PAR in Tween 80 - ammonium sulfate system were studied. A non - organic solvent liquid - solid extraction spectrophotometry was developed for the determination of trace cobalt in biological samples. In p H7.0 ~ 1.05 NH3 · H2 O-NH4 Cl buffer solution, the metal complex Co () -PAR can be Tween 80 solid phase enrichment, the maximum absorption wavelength of 51 5nm, the cobalt content of Beer’s law is obeyed in the range of 0 ~ 5.0 μg / m L. Can be used for determination of trace cobalt in hair, tea leaves and vitamin B12 injections