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目的通过对si RNA-PSMA-LNCa P、PC-3和LNCa P前列腺癌细胞株进行肿瘤转移基因芯片分析,筛选出与前列腺癌细胞转移相关的基因,并对芯片结果进行验证。方法利用肿瘤转移基因芯片技术,对si RNA-PSMALNCa P细胞株、PC-3细胞株和LNCa P前列腺癌细胞株进行肿瘤转移相关基因表达差异分析,从中筛选出可能调控前列腺癌细胞转移的基因,并利用real-time PCR的方法在前列腺特异膜抗原(PSMA)阳性/阴性的前列腺癌细胞株和临床样品中对筛选得到的基因进行进一步验证。结果从肿瘤转移基因芯片结果筛选得到了3种在PSMA表达抑制或不表达情况下显著上调的基因:MMP3、CDH6和MTSS1;3种基因的表达在PSMA阳性细胞株和PSMA阴性细胞株中与芯片结果一致;利用临床组织对肿瘤转移基因芯片结果验证,结果显示3种前列腺癌细胞转移相关基因的表达水平与前列腺癌临床分级和PSMA的表达呈负相关。结论发现了3种可能参与调控前列腺癌细胞转移的基因:MMP3、CDH6和MTSS1。它们的表达水平与前列腺癌临床分级、PSMA的表达呈负相关,可能参与了由PSMA介导的前列腺癌细胞的转移。
OBJECTIVE: To screen for gene transfer related to prostate cancer cell metastasis by microarray analysis of si RNA-PSMA-LNCa P, PC-3 and LNCa P prostate cancer cell lines and validate the results of the chip. Methods Tumor metastasis gene microarray was used to analyze the gene expression of tumor metastasis in si RNA-PSMALNCa P cell line, PC-3 cell line and LNCa P prostate cancer cell line, and to screen out the genes that may regulate the metastasis of prostate cancer cells. And the real-time PCR method was used to further validate the selected genes in prostate-specific membrane antigen (PSMA) positive / negative prostate cancer cell lines and clinical samples. Results Three genes that were significantly upregulated in the presence or absence of PSMA expression were screened from tumor metastasis gene chip results: MMP3, CDH6 and MTSS1. The expression of three genes in PSMA-positive cell line and PSMA-negative cell line was significantly higher than that in chip The results showed that the expression of metastasis-related genes in the three kinds of prostate cancer cells was negatively correlated with the clinical grade of prostate cancer and the expression of PSMA. Conclusions Three genes that may be involved in the regulation of prostate cancer cell metastasis were found: MMP3, CDH6 and MTSS1. Their expression levels were negatively correlated with the clinical grade of prostate cancer and the expression of PSMA, which may be involved in the metastasis of prostate cancer cells mediated by PSMA.