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目的观察生存素(Survivin)反义寡核苷酸(ASODN)对人血管瘤血管内皮细胞凋亡的影响,并初步探讨其作用机制。方法实验于2007年12月至2010年2月完成。以靶向Survivin基因中与Caspase-3结合的部位为模板,设计合成ASODN并通过脂质体将其转染至人血管瘤血管内皮细胞中。MTT观察Survivin ASODN对血管瘤血管内皮细胞增殖的影响;分光光度法检测Caspase-3酶活性。结果 Survivin ASODN可以显著抑制血管瘤血管内皮细胞增殖,而且抑制程度与Survivin ASODN浓度成正比。Survivin ASODN作用于血管瘤血管内皮细胞,Caspase-3酶活性呈时间、剂量依赖性升高。结论以靶向Survivin基因中与Caspase-3结合的部位为模板设计合成的ASODN可显著抑制人血管瘤血管内皮细胞增殖,诱导细胞凋亡,其机制与提高Caspase-3酶活性有关。
Objective To investigate the effect of survivin antisense oligonucleotide (ASODN) on the apoptosis of human hemangioma vascular endothelial cells (VECs) and to explore its possible mechanism. The method experiment was completed from December 2007 to February 2010. ASODN was designed and synthesized by targeting the site of Caspase-3 binding to Survivin gene and transfected into human hemangioma vascular endothelial cells by liposome. The effect of Survivin ASODN on the proliferation of vascular endothelial cells was observed by MTT assay. The activity of Caspase-3 was detected by spectrophotometry. Results Survivin ASODN could significantly inhibit the proliferation of vascular endothelial cells, and the degree of inhibition was directly proportional to the concentration of Survivin ASODN. Survivin ASODN acts on hemangiomas vascular endothelial cells, Caspase-3 activity in a time-and dose-dependent manner. Conclusions ASODN, which is designed to target the site of Caspase-3 binding to Survivin gene, can inhibit the proliferation and induce the apoptosis of human hemangioma vascular endothelial cells. Its mechanism is related to the increase of Caspase-3 activity.