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目的 :建立发育期大鼠高热惊厥脑损伤模型。方法 :采用热水浴诱导大鼠高热惊厥 (febrileconvulsion ,FC) ,隔日诱导惊厥 1次 ,共诱导 10次。发育期大鼠随机分为 3组 :4 5 .0℃热水浴组 (n =6 ) ,4 4 .5℃热水浴组(n =10 ) ,4 4 .0℃热水浴组 (n =10 ) ,选取高热未惊厥与FC比例最合适的一组 ,将此组的水浴温度定为以后实验的高热处理温度。发育期大鼠随机分为两组 :37.0℃水浴正常对照组 (n =10 ) ,4 4 .5℃热水浴组 (n =4 0 ) ,高热处理组又分为高热未惊厥组 (FC =0 ,n =10 )和FC组 (FC≥ 6次 ,n =2 2 )。HE染色观察各组大鼠海马神经元形态学改变 ;尼氏染色观察海马神经元丢失情况 ;电镜观察海马神经元超微结构的改变 ;体视学方法计数海马CA1区神经元数密度。结果 :HE染色可见FC组海马CA1区、CA2区细胞排列紊乱、极向不清、细胞空泡变 ,细胞核大小不一致、圆形或椭圆形 ;尼氏染色未见FC组明显的海马神经元丢失 ;FC组大鼠海马CA1区和门区神经元线粒体体积减少、部分出现空泡、基质浓缩、嵴模糊不清或消失 ,高尔基复合体轻 中度扩张 ;FC组大鼠海马CA1区神经元数密度显著减少 ,与正常对照组及高热未惊厥组比较 ,差异均有显著性。结论 :热水浴诱导大鼠FC与人类FC有许多相似之处 ,大鼠FC频繁发作可导致海马?
Objective: To establish a rat model of developing febrile seizures and brain injury. Methods: Febrileconvulsion (FC) was induced by hot water bath in rats and convulsions were induced one time every other day for 10 times. Developmental rats were randomly divided into three groups: 45 ℃ hot water bath group (n = 6), 44 ℃ hot water bath group (n = 10), 44 ℃ hot water bath group ( n = 10), select the most appropriate group of high fever and convulsion and FC ratio, the water bath temperature in this group as the high heat treatment temperature after the experiment. Rats in development stage were randomly divided into two groups: 37.0 ℃ water bath normal control group (n = 10), 44.5 ℃ hot water bath group (n = 40), high heat treatment group was divided into high fever non-convulsion group (FC = 0, n = 10) and FC group (FC≥ 6 times, n = 2 2). The morphological changes of hippocampal neurons in each group were observed by HE staining. The loss of hippocampal neurons was observed by Nissl staining. The ultrastructure of hippocampal neurons was observed by electron microscopy. The number density of neurons in hippocampus CA1 was counted by stereological method. Results: HE staining showed that hippocampal CA1 and CA2 cells in the hippocampus were disordered, depolarized, devoid of vacuolization, inconsistent nucleus size, round or oval in the hippocampus of FC group, and no obvious hippocampal neuron loss in FC group . The mitochondria of hippocampal CA1 region and portal region in FC group were decreased in volume, vacuolized, matrix was concentrated, the cristae were blurred or disappear, and the Golgi complex was mildly and moderately expanded. The number of neurons in hippocampal CA1 region of FC group The density was significantly reduced, compared with the normal control group and hyperthermia without convulsion group, the difference was significant. CONCLUSION: There are many similarities between hot water bath-induced FC and human FC in rats. Frequent episodes of FC in rats may lead to hippocampus?