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[目的]旨在明确新型生防细菌贝莱斯芽孢杆菌S6的拮抗物质和抑菌作用。[方法]拮抗物质的研究方法包括硫酸铵沉淀法、DEAE-Sephrose~(TM) Fast Flow阴离子交换、Superdex TM75凝胶层析及SDS-PAGE电泳分析;抑菌作用的研究方法包括菌丝生长速率法和孢子萌发法。[结果]贝莱斯芽孢杆菌S6拮抗物质对多种病原菌具有很好的抑制作用。采用80%饱和度(NH_4)_2SO_4沉淀法提取贝莱斯芽孢杆菌S6的发酵液粗提物,粗提物通过DEAE-Sephrose TM Fast Flow阴离子交换和Superdex~(TM) 75凝胶层析进行纯化,经SDS-PAGE电泳检测到单一的条带,其分子质量为20.1 kDa。贝莱斯芽孢杆菌S6的拮抗蛋白质量浓度为5、10 g/L时,对番茄早疫病菌的抑制率分别为71.13%、90.33%;质量浓度为1 g/L时,对早疫病菌的孢子萌发抑制率为60.42%,质量浓度达到10 g/L时,孢子几乎不萌发。拮抗蛋白处理的菌丝发生了扭曲、畸形、肿大。[结论]本研究结果为利用贝莱斯芽孢杆菌S6开发新的生防制剂奠定了基础。
[Objective] The purpose of this study was to clarify the antagonistic substances and antibacterial activity of a new biocontrol bacterium Bacillus spp. S6. [Method] The research methods of antagonistic substances include ammonium sulfate precipitation, DEAE-Sephrose Fast Flow anion exchange, Superdex TM75 gel chromatography and SDS-PAGE electrophoresis. The research methods of bacteriostasis include mycelial growth rate Law and spore germination method. [Result] The antagonistic substance of Berezisi spp. S6 had a good inhibitory effect on many pathogens. Crude extracts of B.cerevisiae S6 fermentation broth were extracted by 80% saturation (NH 4) 2 SO 4 precipitation and the crude extracts were purified by DEAE-Sephrose ™ Fast Flow anion exchange and Superdex ™ 75 gel chromatography , A single band was detected by SDS-PAGE electrophoresis and its molecular mass was 20.1 kDa. When the concentration of antagonistic protein of Berezis bacterium S6 was 5 and 10 g / L, the inhibitory rates on A. solani were 71.13% and 90.33%, respectively. When the concentration of 1 g / L was 1 g / L, Spore germination inhibition rate of 60.42%, mass concentration of 10 g / L, the spores almost germination. Antagonistic protein processing mycelium twisted, deformity, enlargement. [Conclusion] The results of this study laid the foundation for the development of a new biocontrol agent by using Berezis S6.