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作者以采自皮下结节的盘尾丝虫的雌雄成虫及采自库蚊体内的班氏丝虫第三期幼虫作冰冻切片后固定于玻片上作为抗原,进行间接萤光抗体试验。呈现明显萤光的血清最高稀释度在1:64或以上者作为阳性标准。105例经确诊的盘尾丝虫病人的血清对同种抗原呈阳性反应的有102例(97%),对班氏丝虫抗原的阳性率为86%(19/22)。44例班氏丝虫病人对同种抗原有33例(75%)呈阳性反应。其中有急性症状的8例,不论血中有无微丝蚴,反应均为阳性;28例有慢性症状(象皮肿或淋巴水肿)而无微丝蚴血症者有22例(79%)阳性;无症状而有微丝血症的8例中仅3例阳性,该类病例的特异性抗体最
In this study, the indirect-fluorescent antibody test was performed on male and female adults of Onchocerca spp. Collected from subcutaneous nodules and third-stage larvae of Bancroftian filaments collected from Culex mosquitoes as frozen sections and fixed on glass slides. Fluorescent serum showed a maximum dilution of 1:64 or above as a positive standard. Serum samples of 105 confirmed cases of Onchocerciasis were positive for alloantigens in 102 (97%) and 86 (19/22) cases. 44 cases of Bancroftian filarial patients with alloantigens 33 cases (75%) were positive. Eight of the patients with acute symptoms had a positive reaction, regardless of the presence or absence of microfilaria in the blood. Twenty-eight patients (79%) had chronic symptoms (elephantiasis or lymphedema) without microfilariaemia in 28 patients Positive; asymptomatic and micro-hyperlipidemia in only 8 cases were positive, the most specific antibodies in these cases