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用限制性内切酶ＥｃｏＲＩ和ＳａｌＩ将恶性疟原虫复合抗原基因ＰｆＣＭＲ从质粒ｐＷＲ４５０－１／ＰｆＣＭＲ中切下，插入质粒ｐＢＶ２２０／ＩＬ－２中人白细胞介素－２（ＩＬ－２）基因的ＥｃｏＲＩ位点。重组质粒转化大肠杆菌ＤＨ５ａ，通过ＰＣＲ扩增和酶切鉴定，筛选出正向插入的重组载体ｐＢＶ２２０／ＰｆＣＭＲ－ＩＬ－２。为表达ＰｆＣＭＲ－ＩＬ－２融合蛋白打下基础。
Plasmodium falciparum complex antigen gene PfCMR was excised from plasmid pWR450-1 / PfCMR with restriction enzymes EcoRI and SalI and inserted into Eco RI (R) of human interleukin-2 (IL-2) gene in plasmid pBV220 / IL- Site. The recombinant plasmid was transformed into E. coli DH5a and identified by PCR amplification and restriction enzyme digestion. The recombinant vector pBV220 / PfCMR-IL-2 was screened out. Lay the foundation for expressing PfCMR-IL-2 fusion protein.