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应用高香草酸荧光分析技术及NADH-高铁氰化钾还原酶法,对正常和Graves病甲状腺过氧化氢（H2O2）和NADH-细胞色素b5还原酶（b5R）进行测定,发现Graves病甲状腺b5R活性和H2O2水平均明显高于正常,而H2O2酶活性在Graves病和正常甲状腺间无显著差异。加b5R抑制剂对氯汞苯甲酸抑制b5R活性,Graves病和正常甲状腺b5R活性降低近85％,同时H2O2降低近50％,蛋白结合碘形成减少近52％。b5R活性和H2O2水平两者呈显著正相关关系。以上结果表明,b5R参与甲状腺内H2O2的生物合成,是甲状腺内产生H2O2的重要酶系。
High vanillic acid fluorescence analysis and NADH-ferricyanide reductase method were used to measure thyroid hydrogen peroxide (H2O2) and NADH-cytochrome b5 reductase (b5R) in normal and Graves’ disease. The results showed that b5R activity of thyroid in Graves disease And H2O2 levels were significantly higher than normal, and H2O2 activity in Graves disease and normal thyroid no significant difference. Inhibition of b5R activity by b5R inhibitors of clorp mercuric acid reduced the activity of b5R by nearly 85% in Graves’ disease and in normal thyroid glands, while H2O2 decreased by nearly 50% and protein-bound iodine formation by approximately 52%. There was a significant positive correlation between b5R activity and H2O2 level. The above results show that b5R is involved in the biosynthesis of H2O2 in the thyroid gland and is an important enzyme producing H2O2 in the thyroid gland.