论文部分内容阅读
目的:分离纯化到单一组分的木鳖子素。方法:木鳖子脱脂粉用磷酸盐缓冲液提取,提取液用硫酸铵沉淀,沉淀用水溶解后先上CM Sepharose Fast F low阳离子交换凝胶柱然后上SephacrylTMS-200 H igh F low凝胶柱。结果:纯化到单一组分的木鳖子素,分子量为29000。结论:该方法快速、稳定。
Purpose: Separation and purification of single component of Hirudin. Method: The defatted powder of wolfberry fruit was extracted with phosphate buffer, and the extract was precipitated with ammonium sulfate. The precipitate was dissolved in water and then applied on a CM Sepharose Fast Low cation exchange gel column and then on a Sephacryl TMS-200 H igh F low gel column. RESULTS: The purified quercetin with a single component had a molecular weight of 29,000. Conclusion: This method is fast and stable.