目的 :研究人胚肺 (HEL)细胞HOXB1,HOXB5 ,HOXB6及HOXB9基因的表达状态及人类巨细胞病毒(HCMV)感染对上述基因表达的影响。方法 :采用半定量逆转录 -聚合酶链反应 (RT -PCR)技术。结果 :①HEL细胞表达HOXB5和HOXB6 ,但不表达HOXB1和HOXB9;②HCMV感染后 ,HEL细胞HOXB6基因的表达上调 ,且被HCMV诱导表达HOXB9T ,而HOXB5基因的表达无明显改变 ,HOXB1基因仍旧不表达 ;③全反式维甲酸 (ATRA)能上调HCMV感染后HOXB9基因的表达 ,但对HCMV感染晚期HOXB6基因的表达有抑制作用。结论 :HCMV能诱导HOXB6和HOXB9基因表达异常 ,这可能在先天性HCMV感染导致胚胎发育畸形中起重要作用 Objective: To investigate the expression of HOXB1, HOXB5, HOXB6 and HOXB9 in human embryo lung (HEL) cells and the effect of human cytomegalovirus (HCMV) infection on the above gene expression. Methods: Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used. Results: ①HEL cells expressed HOXB5 and HOXB6 but did not express HOXB1 and HOXB9; ② HOXB6 gene expression was up-regulated in HEL cells after HCMV infection, and HOXB9T was induced by HCMV, but HOXB5 gene expression was not changed but HOXB1 gene was still not expressed; All-trans retinoic acid (ATRA) can up-regulate the expression of HOXB9 gene after HCMV infection, but inhibit the expression of HOXB6 gene late in HCMV infection. Conclusion: HCMV can induce abnormal gene expression of HOXB6 and HOXB9, which may play an important role in embryonic developmental deformity caused by HCMV infection