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本文应用ＨＰＶ－ＰＣＲ技术和ＤＮＡ杂交技术对７６例宫颈鳞癌标本进行了ＨＰＶ－ＤＮＡ的研究，发现ＨＰＶ－ＤＮＡ存在率为９０．７９％（６９／７６），主要类型为ＨＰＶ１６和１８。多酶切ＳｏｕｔｈｅｒｎＢｌｏｔ杂交证实在宫颈癌中９２．５６％的ＨＰＶ－ＤＮＡ发生变异，说明ＨＰＶ－ＤＮＡ的基因变异与其致癌作用密切相关。ＰＣＲ结果证实ＨＰＶ－Ｅ６片段在宫颈癌组织中常可出现，推测可能是致癌的关键片段之一。因此，检测宫颈癌组织中ＨＰＶ－ＤＮＡ的完整性、关键基因片段的存在与否，可能对于病变恶变的预测有一定的意义
HPV-DNA was detected in 76 cases of cervical squamous cell carcinoma using HPV-PCR technology and DNA hybridization technology. The HPV-DNA was found in 90.79% (69/76) of the HPV-DNA samples and the main types were HPV16 and HPV18. Multiple Southern blot analysis confirmed 92.56% of HPV-DNA mutations in cervical cancer, indicating that HPV-DNA genetic variation is closely related to its carcinogenicity. PCR results confirmed that HPV-E6 fragments often appear in cervical cancer tissue, presumably one of the key fragments of carcinogenesis. Therefore, the detection of HPV-DNA in cervical cancer integrity, the presence or absence of key gene fragments may predict the malignant lesions of a certain significance