用含有抗除草剂溴苯腈与雄性不育嵌合基因、抗除草剂溴苯腈与育性恢复嵌合基因转化油菜下胚轴组织，分化再生获得转基因雄性不育植株和育性恢复植株。用除草剂溴苯腈对转基因雄性不育植株和育性恢复植株进行抗性鉴定，抗性可达１０－３ｍｏｌ／Ｌ。再以抗溴苯腈基因为探针分别对其进行Ｓｏｕｔｈｅｒｎ杂交分析，杂交结果均为阳性。对两种植株花器解剖表明，雄性不育植株的花丝明显比花柱短，花粉少，育性恢复植株与正常植株相同 Transgenic male sterile plants and fertility restorer plants were obtained by transformation of the hypocotyl tissue of rape with the chloramphenicol and male sterility chimeric genes containing the herbicide bromoxynil and fertility restorer chimeric genes. The herbicide bromoxynil was used to evaluate the resistance of transgenic male sterile plants and fertility restorer plants with resistance up to 10-3mol / L. Then, the anti-bromoxynil gene was used as a probe for Southern hybridization and the result of hybridization was positive. An anatomical study on floral organs of the two plants showed that the filaments of the male-sterile plants were significantly shorter than the styles and pollen was less, and the fertility-restoring plants were the same as the normal plants