鼓膜穿孔是临床常见病及多发病,穿孔的修复机制、鼓膜愈合的影响因素以及修复材料的研究都离不开合适的鼓膜穿孔模型。急性鼓膜穿孔大多可自愈,慢性鼓膜穿孔一般指穿孔迁延2个月以上尚未愈合者,急性鼓膜穿孔的动物模型并不适合于评价不同材料修补鼓膜的优劣〔1〕。本实验在鼓膜 Perforation of the tympanic membrane is a common clinical disease and frequently-occurring disease, perforation repair mechanism, the factors affecting the tympanic membrane healing and repair of materials are inseparable from the appropriate tympanic membrane perforation model. Most of the acute tympanic membrane perforation can be self-healing, chronic tympanic membrane perforation generally refers to the perforation delayed more than 2 months have not healed, acute tympanic membrane perforation animal model is not suitable for evaluating the pros and cons of different materials to repair the tympanic membrane 〔1〕. The experiment in the tympanic membrane