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目的:建立兔慢性肝纤维化胆石病模型,研究大黄灵仙颗粒对慢性肝纤维化胆石病兔模型的干预作用。方法:参考既往造模方法进行动物模型构建并进行中药制剂大黄灵仙颗粒干预,用药12周后,麻醉后耳缘静脉采血并取肝脏组织,测定肝组织羟脯氨酸含量,HE染色观察肝脏组织病理变化及检测ALT、AST、TBIL、DBIL、IBIL、GHO、TG。结果:与对照组相比,治疗组血清ALT、AST、TBIL、DBIL、IBIL、GHO、TG等生化指标均较前明显改变(均为P<0.05);与对照组相比,治疗组肝组织羟脯氨酸含量均明显下降(P<0.01);病理显示治疗组光镜下可见遭到破坏的肝组织逐渐修复,肝细胞排列略变规则,水肿肝细胞明显减少,炎性细胞及纤维组织减少,区域呈点状、片状坏死明显减少。结论:大黄灵仙颗粒能防治慢性肝纤维化胆石病,具体效应机制有待探索。
Objective: To establish a model of cholelithiasis in rabbits with chronic liver fibrosis and study the effect of rhubarb and Lingxian granule on the model of chronic liver fibrosis gallbladder in rabbits. Methods: The animal models were constructed according to the previous methods and the Chinese medicine rhubarb granules was intervened. After 12 weeks of treatment, the blood was drawn from the ear vein and the liver tissue was taken for measurement of hydroxyproline content in the liver tissue. HE staining was used to observe the liver Histopathological changes and detection of ALT, AST, TBIL, DBIL, IBIL, GHO, TG. Results: Compared with the control group, the serum ALT, AST, TBIL, DBIL, IBIL, GHO, TG and other biochemical indexes were significantly changed (all P <0.05) (P <0.01). The pathology showed that the damaged liver tissue was gradually repaired by light microscopy in the treatment group, the arrangement of hepatocytes was slightly changed, the number of edematous hepatocytes was significantly decreased, the number of inflammatory cells and fibrous tissue Reduce, the region was punctate, significantly reduced sheet necrosis. Conclusion: Rhubarb granules can prevent and treat chronic hepatic fibrosis gallbladder disease, the specific mechanism needs to be explored.