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目的 :揭示V毒素在溶血性尿毒症 (HUS)和其他与多源性大肠杆菌 (VTEC)相关疾病中的病理性损伤作用。方法 :运用流式细胞技术 ,荧光标记探针———BCECF AM和细胞色素C还原法测定超氧离子 (O- 2 )技术。结果 :重组V2毒素 (rVT2 )对中性粒细胞自发性凋亡有着显著性的抑制作用。被rVT2 延迟了凋亡的中性粒细胞仍然保留多种生物学活性 ,如 :细胞表面粘附分子的表达 (CD6 2L减少和CD11b CD18增加 ) ,粘附人脐带静脉血管内皮细胞 (HUVEC)以及产生超氧离子 (O-2 )。结论 :V2毒素延长中性粒细胞的功能性生命期限 ,会加重炎症局部的炎症反应 ,这可能是HUS和VTEC相关疾病的发生过程中 ,中性粒细胞介导的组织损伤的一个重要因素。
OBJECTIVE: To reveal the pathological lesion of V toxin in hemolytic uremia (HUS) and other diseases associated with multi-source Escherichia coli (VTEC). Methods: The technique of flow cytometry (FCM) was used to detect the superoxide ion (O 2) by fluorescence labeled probe BCECF AM and cytochrome C reduction. Results: Recombinant V2 toxin (rVT2) had a significant inhibitory effect on spontaneous apoptosis of neutrophils. Neutrophils that are delayed in apoptosis by rVT2 still retain a variety of biological activities such as cell surface adhesion molecule expression (reduced CD6 2L and increased CD11b CD18), adhesion to human umbilical vein endothelial cells (HUVECs), and Produces superoxide ion (O-2). CONCLUSION: V2 toxin prolongs the functional life span of neutrophils, which may aggravate the inflammatory response in the inflammatory area. This may be an important factor in neutrophil-mediated tissue damage during the development of HUS and VTEC-related diseases.