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目的 :探讨导向化疗对肠癌细胞增殖动力学的影响。方法 :5 0例大肠腺癌患者随机分成三组 :A组单纯手术切除 ;B组术前静注化疗药物 ;C组术前静注导向化疗药物。术中取肿瘤组织及周围正常肠道组织进行AgNORs染色及检测。 结果 :1 手术组 (2 0例 )、化疗组 (13例 )、导向化疗组 (17例 )癌细胞AgNORs/核颗粒分别为4 18± 0 5 0、3 13± 0 2 0、2 2 9± 0 6 9,两两比较其差异存在显著性 (P <0 0 0 1) ;正常肠道细胞AgNORs/核均数分别为 1 81± 0 32、1 86± 0 2 5、1 80± 0 33,(P >0 0 5 )。 2 手术组、化疗组、导向化疗组肿瘤细胞AgNORs颗粒面积与细胞核面积的比值分别为 :0 2 85 8± 0 0 6 74、0 2 0 2 6± 0 0 5 93、0 2 0 0 9± 0 0 35 5 ,化疗组、导向化疗组与手术组比较其差异有显著性 (P <0 0 0 1) ,化疗组与导向化疗组之间的差异无显著性 (P >0 0 5 )。 3 手术组、化疗组、导向化疗组肿瘤细胞AgNORs颗粒光密度与肿瘤细胞核总光密度比值分别为 0 36 6 8± 0 0 6 0 3、0 30 36± 0 0 5 18、0 2 5 12± 0 0 374,两两比较差异有显著性 (P <0 0 1)。结论 :导向化疗与化疗均影响大肠癌细胞的增殖动力学 ,导向化疗对癌细胞增殖动力学的影响比化疗更明显。
Objective: To investigate the effect of targeted chemotherapy on the proliferation kinetics of colon cancer cells. Methods : A total of 50 patients with colorectal adenocarcinoma were randomly divided into three groups: Group A was treated by surgery alone; Group B was given intravenous chemotherapy before surgery; Group C was given preoperative intravenous injection of chemotherapy. AgNORs staining and detection were performed during the operation to obtain tumor tissue and surrounding normal intestinal tissues. RESULTS: 1 The AgNORs/nuclear granules in the surgical group (20 cases), the chemotherapy group (13 cases), and the directional chemotherapy group (17 cases) were 4 18±0 5 0, 3 13± 0 20, 2 2 9 ± 0 6 9, the difference between the two groups was significant (P <0 0 0 1); AgNORs/nucleus averages of normal intestinal cells were 1 81 ± 0 32, 1 86 ± 0 2 5, and 1 80 ± 0, respectively. 33, (P > 0 0 5). 2 The ratio of AgNORs and nuclei area of tumor cells in surgical group, chemotherapy group, and chemoradiotherapy group were: 0 2 85 8± 0 0 6 74, 0 2 0 2 6± 0 0 5 93, 0 2 0 0 9 ± 0 0 35 5 , there was a significant difference between the chemotherapy group, the chemotherapy group and the surgery group (P 0101), and there was no significant difference between the chemotherapy group and the chemotherapy group (P 0 05). 3 The ratio of AgNORs granule optical density and tumor nuclei total optical density of tumor cells in surgical group, chemotherapy group, and chemoradiotherapy group were 0 36 68 ± 0 0 6 0 3, 0 30 36 ± 0 0 5 18, 0 2 5 12 ± 0 0 374, there was a significant difference between the two (P <0 01). Conclusion : Both chemotherapy and chemotherapy-guided chemotherapy affect the proliferation kinetics of colorectal cancer cells. The effect of targeted chemotherapy on proliferation kinetics of cancer cells is more pronounced than chemotherapy.