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以改性磁性壳聚糖为载体,四环素(TC)为模板分子,采用表面聚合法制备磁性四环素分子印迹材料(MIP).用FTIR对MIP进行表征.对MIP进行吸附性能研究,结果表明:MIP对TC的最大吸附量为56.60 mg/g;吸附平衡时间为1.5h,且主要吸附过程由化学吸附控制;MIP对四环素类抗生素(TCs)具有良好的组选择性.同时以该印迹聚合物为固相萃取材料,建立了MISPE-HPLC分析方法,用于猪肉和蜂蜜样品中TCs残留分析,回收率范围84.0％ ～99.3％,RSD小于4.0％.所制备的MIP对样品中的TCs的分离富集效果良好.“,”The magnetic molecularly imprinted polymer (MIP) was prepared by surface polymerization using tetracycline (TC) as template and the magnetic-chitosan as supporter.The structure and properties of MIP were characterized by FTIR and adsorption experiments.The results showed that the maximum adsorption capacity of MIP for TC was 56.60 mg/g.The adsorption equilibrium time was 1.5 h,and the main adsorption process was controlled by chemical adsorption.MIP also exhibited good group-selective for tetracycline antibitics (TCs).Meanwhile the MISPE-HPLC method was developed using MIP as the solid phase extraction material for the residual analysis of TCs in the pork and honey samples.The recovery was in range of 84.0％-99.3％,and RSD was less than 4.0％.The obtained MIP showed high efficiency on the separation and enrichment of TCs in the various samples.