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目的:研究热休克蛋白70(HSP70)与MHC-I类抗原(HLA-A/B/C)在人原发性肝细胞癌(HCC)组织中的表达并探讨二者与肿瘤生物学行为的关系。 方法:采用免疫组织化学方法检测了10例正常肝组织、40例HCC肿瘤组织及癌旁组织中HSP70及MHC-I类抗原的表达。 结果:HSP70在正常肝脏、癌旁组织、HCC中的阳性表达率分别为10％(1/10)、45％(18/40)、80％(32/40),HCC中的阳性表达率明显高于癌旁组织和正常肝脏(x_1~2=10.45,x_2~2=14.49,P<0.05)。HLA-A/B/C在正常肝脏、癌旁组织、HCC中的阳性表达率分别为100％(10/10)、95％(38/40)、55％(22/40),其在癌组织阳性表达率明显低于癌旁组织和正常肝脏组织(X_1~2=5.21,x_2~2=17.06,P<0.05)。HSP70表达与癌周淋巴细胞浸润(x~2=1.4,P>0.05)和转移(x~2=1.9,P>0.05)无关,但与癌组织分化程度有关,HSF70在高、中、低分化肝癌中的阳性表达率分别为50％(5/10)、78.6％(11/14)、100％(16/16),中、低分化肝癌中的阳性表达率高于高分化肝癌(X~2=9.40,P<0.05)。HSP70+HSP70+HLA-A/B/C+者,高、中、低分化HCC所占比例分别为22.2％(4/18)、50％(9/18)、27.8％(5/18)。HSP70与HLA-A/B/C其一或二者均为阴性时,高、中、低分化HCC所占比例分别为18.2％(4/22)、22.7％(5/22)、59.1％(13/22)。前者分化?
OBJECTIVE: To study the expression of heat shock protein 70 (HSP70) and MHC class I antigen (HLA-A / B / C) in human primary hepatocellular carcinoma (HCC) and to explore their relationship with the biological behavior of tumor relationship. Methods: Immunohistochemistry was used to detect the expression of HSP70 and MHC class I antigen in 10 normal liver tissues, 40 HCC tumor tissues and adjacent tissues. Results: The positive rates of HSP70 expression in normal liver, paracancer tissue and HCC were 10% (1/10), 45% (18/40) and 80% (32/40), respectively. The positive rate of HSP70 in HCC was significantly higher than that in normal liver Which was higher than that in adjacent tissues and normal liver (x_1 ~ 2 = 10.45, x_2 ~ 2 = 14.49, P <0.05). The positive rates of HLA-A / B / C in normal liver, paracancerous tissue and HCC were 100% (10/10), 95% (38/40) and 55% (22/40) The positive expression rate of tumor tissue was significantly lower than that in paracancerous tissues and normal liver tissues (X_1 ~ 2 = 5.21, x_2 ~ 2 = 17.06, P <0.05). The expression of HSP70 was not correlated with the degree of peritumoral lymphocytosis (x ~ 2 = 1.4, P> 0.05) and metastasis (x ~ 2 = 1.9, P> 0.05) The positive expression rates in HCC were 50% (5/10), 78.6% (11/14) and 100% (16/16), respectively. The positive rate of HCC in HCC was higher than that in HCC 2 = 9.40, P <0.05). HSP70 + HSP70 + HLA-A / B / C +, high, moderate and poorly differentiated HCC accounted for 22.2% (4/18), 50% (9/18), 27.8% (5/18) respectively. The rates of high, moderate and poorly differentiated HCC were 18.2% (4/22), 22.7% (5/22), 59.1% (5/22), respectively, when both HSP70 and HLA-A / B / 13/22). The former differentiation?