论文部分内容阅读
研究了硫堇(TH)的同步荧光光谱(△λ=10nm),消除了瑞利散射的干扰,并确定λ_(ex)=624nm,λ_(em)=634nm。进一步观察了Fe~(3+)对硫堇同步荧光的猝灭作用,并在同步荧光猝灭度(△F)与Fe~(3+)质量浓度(pFe~(3+))之间建立了良好的线性关系(△F=536.3p、r=0.9986)。Fe~(3+)的线性范围0.0283—0.890μg·mL~(-1),检出限为0.0085μg·mol~(-1),加标回收率为96.7%—104.6%。该方法选择性高,稳定性好,用于3种不同啤酒样品中的痕量铁的测定,结果满意。
Simultaneous fluorescence spectra (Δλ = 10 nm) of thionine (TH) were studied, and the interference of Rayleigh scattering was eliminated, and λ ex was determined to be 624 nm and λ em = 634 nm. The quenching effect of Fe ~ (3+) on simultaneous fluorescence of thionine was further observed and established between the synchronous fluorescence quenching (△ F) and the concentration of Fe 3+ (pFe ~ (3+)) A good linear relationship (△ F = 536.3p, r = 0.9986). The linear range of Fe 3+ was 0.0283-0.890μg · mL -1, the detection limit was 0.0085μg · mol -1, and the recoveries were 96.7% -104.6%. The method has high selectivity and good stability, and is suitable for the determination of trace iron in three different beer samples with satisfactory results.