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【目的】对寄生茶小绿叶蝉的丝状真菌进行鉴定和分生孢子培养研究。【方法】采用形态特征比较和转录间隔区(ITS)序列构建系统树进行分析,分生孢子培养通过单因素筛选和正交试验进行产孢条件优化。【结果】根据形态特征比较和系统发育分析表明,该真菌为渐狭蜡蚧菌Lecanicillium attenuatum Zare&W.Gams。最优产孢条件(质量体积比)为:蛋白胨2%,麦芽糖1%,蚕蛹粉1%,氯化钾0.05%,磷酸氢二钾0.1%,七水硫酸镁0.05%,琼脂1.5%,蒸馏水1 000 m L,25°C。【结论】通过形态特征比较和分子序列分析表明,罹病茶小绿叶蝉上的真菌为已知种——渐狭蜡蚧菌,并对该菌株进行了产孢条件的优化。研究结果为该菌株应用到茶小绿叶蝉的生物防治研究提供基础资料。
【Objective】 The objective of this study was to identify filamentous fungi of the parasitoid Tea Leafhopper and its conidial culture. 【Method】 The phylogenetic tree was used to analyze the morphological traits and ITS sequences. The conidiospore culture was optimized by single factor screening and orthogonal experiment. 【Result】 According to morphological characteristics and phylogenetic analysis, the fungus was Lecanicillium attenuatum Zare & W. Gams. The optimum conditions of spore production were as follows: peptone 2%, maltose 1%, silkworm pupa powder 1%, potassium chloride 0.05%, potassium hydrogen phosphate 0.1%, magnesium sulfate heptahydrate 0.05%, agar 1.5% 1 000 m L, 25 ° C. 【Conclusion】 Morphological comparison and molecular sequence analysis showed that the fungus on the diseased Tea Green Leafhopper was a known species, Meloidogyne spp., And its sporulation conditions were optimized. The results provide the basic data for the biological control of this strain.