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目的探讨锰染毒大鼠空间学习记忆变化的规律。方法50只雄性大鼠适应性喂养1周后,随机分为5组,即生理盐水组(A组),Mn2+2.5,5,10,20 mg/kg(B、C、D、E组)。腹腔注射生理盐水或MnCl2溶液0.5 mL/d,共30 d。染毒结束后,采用Morris水迷宫试验测定大鼠潜伏期、总路程和穿台次数的变化。结果经多组比较的单因素方差分析,潜伏期、总路程、穿台次数各组均数间差异有统计学意义(F=7.813,P=0.000;F=8.190,P=0.000;F=3.461,P=0.000);与A组比较,B、C、D和E组潜伏期延长,差异有统计学意义(P<0.05);与B组比较,D和E组潜伏期延长,差异有统计学意义(P<0.05)。与A组比较,C、D和E组总路程延长,差异有统计学意义(P<0.01);与B组比较,C、D和E组总路程延长,差异有统计学意义(P<0.01)。与A组比较,B、C、D、E 4组穿台次数减少,差异有统计学意义(P<0.05)。结论锰染毒可以引起大鼠空间学习记忆障碍。
Objective To investigate the changes of spatial learning and memory in manganese-exposed rats. Methods Fifty male rats were fed one week after their adaptive feeding. The rats were randomly divided into 5 groups: normal saline group (group A), Mn2 + 2.5, 5, 10, 20 mg / kg group B, . Intraperitoneal injection of normal saline or MnCl2 solution 0.5 mL / d, a total of 30 d. After the end of the exposure, the Morris water maze test was used to measure the changes of the latency, the total distance and the number of wearing the rats. Results One-way analysis of variance (ANOVA) showed that there was significant difference between the mean of each group (F = 7.813, P = 0.000; F = 8.190, P = 0.000; P = 0.000). Compared with group A, the latency in groups B, C, D and E was longer (P <0.05), and the latency in groups D and E was longer than that in group B P <0.05). Compared with group A, the total distance of group C, D and E was longer than that of group A (P <0.01). Compared with group B, the total distance of group C, D and E was longer (P <0.01) ). Compared with group A, the number of wearing tracts in groups B, C, D and E 4 decreased, with a significant difference (P <0.05). Conclusion Manganese exposure can cause spatial learning and memory impairment in rats.