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以处于性腺发育期为Ⅲ期、IV期和V期的河川沙塘鳢(Odontobutis potamophila)雌鱼为研究对象,采用碱不稳定性蛋白结合磷法测定该鱼肝脏、血清和卵巢中的磷含量,以磷的含量来反应卵黄蛋白原的含量。结果显示,在河川沙塘鳢雌鱼的性成熟过程中,肝脏中的VTG含量先升后降,在IV期达到最高,其含量为(39.91±3.80)mg.g-1,在Ⅲ期和Ⅳ期,VTG合成比较活跃;血清中的VTG含量逐渐增加,从Ⅲ期的(15.45±1.77)mg.mL-1增加到V期的(33.31±5.57)mg.mL-1;卵巢中的VTG含量也逐渐增加,Ⅲ期到Ⅳ期增长速度较快(p<0.05),是VTG沉积的最重要时期,V期达到高峰,为(422.25±29.98)mg.g-1。研究揭示河川沙塘鳢性成熟过程中,VTG的合成活跃,转运高效,为其大量沉积奠定基础,保证了胚胎发育及后续发育的营养需要。
To investigate the effects of alkali-labile protein and phosphorus on the phosphorus content in the liver, serum and ovary of female Pseudoras nobilis (Pomodorophyta) from stage Ⅲ, stage IV and stage V of gonadal development, , The content of phosphorus to reflect the content of vitellogenin. The results showed that during the sexual maturation process, the content of VTG in the liver increased first and then decreased, reaching the highest level in IV stage (39.91 ± 3.80) mg.g-1, The level of VTG in serum increased gradually from (15.45 ± 1.77) mg.mL-1 in the third phase to (33.31 ± 5.57) mg.mL-1 in the V phase, and VTG in the ovary (P <0.05). It is the most important period for the deposition of VTG. The peak of V phase is (422.25 ± 29.98) mg.g-1. The study revealed that in the process of sexual maturation, the synthesis of VTG was active and translocated efficiently, laying a solid foundation for its massive deposition and guaranteeing the nutritional needs of embryo development and subsequent development.