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目的观察４月龄和７月龄胎肝提取物（Ｅ４，Ｅ７）对ＢＥＬ７４０２肝癌细胞增殖和分化的影响．方法采用显微镜下细胞计数，３ＨＴｄＲ掺入和ＭＴＴ比色法，观察（１０～１０００）ｍｇ／Ｌ的Ｅ４和Ｅ７对培养中ＢＥＬ７４０２肝癌细胞的影响．结果Ｅ４从１００ｍｇ／Ｌ起可明显抑制７４０２细胞的生长和ＤＮＡ合成，细胞生长抑制ＩＣ５０为１４４４ｍｇ／Ｌ，ＤＮＡ合成抑制ＩＣ５０为２２７９ｍｇ／Ｌ．在（２５０～１０００）ｍｇ／Ｌ时对整体细胞线粒体脱氢酶活力有明显的抑制作用，ＩＣ５０为１０１３０ｍｇ／Ｌ．Ｅ７在（１０～１００）ｍｇ／Ｌ对细胞生长和ＤＮＡ合成具有明显的促进作用，至１０００ｍｇ／Ｌ浓度未发现明显的抑制作用．在５００ｍｇ／Ｌ和１０００ｍｇ／Ｌ浓度时对整体线粒体脱氢酶活力具有一定的抑制作用．对细胞生长曲线影响，Ｅ４表现为５００ｍｇ／Ｌ时能明显抑制７４０２细胞的生长，而在５０ｍｇ／Ｌ时影响不明显；Ｅ７表现为５００ｍｇ／Ｌ时影响不明显，而５０ｍｇ／Ｌ时则有明显的促进作用．（１００～１０００）ｍｇ／Ｌ的Ｅ４能明显促进单个细胞脱氢酶的活力，而Ｅ７只在１０００ｍｇ／Ｌ时有一定的促进作用，在（１０～５０）ｍｇ／Ｌ对额定细胞脱氢酶?
Objective To observe the effects of fetal liver extracts (E4, E7) at 4 months and 7 months on the proliferation and differentiation of BEL-7402 hepatoma cells. Methods Microscopic cell counting, 3HTdR incorporation and MTT colorimetry were used to observe the effects of (10–1000) mg/L E4 and E7 on BEL7402 hepatoma cells. Results E4 could inhibit the growth and DNA synthesis of 7402 cells from 100 mg/L. The IC50 of cell growth inhibition was 1444mg/L, and the DNA synthesis inhibition IC50 was 2279mg/L. In the range of (250-1000) mg/L, the mitochondrial dehydrogenase activity of the whole cells was significantly inhibited. The IC50 was 10130mg/L. E7 (10～100) mg/L had obvious promoting effect on cell growth and DNA synthesis, and no significant inhibitory effect was observed at 1000 mg/L. The concentration of 500mg/L and 1000mg/L had a certain inhibitory effect on the mitochondrial dehydrogenase activity. The influence of cell growth curve, E4 performance of 500mg/L can significantly inhibit the growth of 7402 cells, but the effect is not obvious at 50mg/L; E7 performance is not obvious at 500mg/L, and 50mg/L is obvious The promotion effect. (100-1000) mg/L of E4 can significantly promote the activity of single cell dehydrogenase, while E7 only has a promoting effect at 1000 mg/L, and (10-50) mg/L is rated for cell dehydrogenase. ?